大鼠脂联素ELisayabo官网手机版 盒,BR

中文名称： 大鼠脂联素检测yabo官网手机版 盒
英文名称： Rat Adiponectin ELISA Kit
别名： ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28apM1; Gelatin-binding protein
储存条件： 2-8℃，6 months
规格： 96T ; 48T
检测目标： Adiponectin
样品类型： 血清/血浆/细胞培养上清
反应性： Rat
应用： ELISA
反应类型： Sandwich-ELISA
定量/定性： 定量检测
检测范围： 43.75-2800 pg/ml
灵敏度： 21.75 pg/ml
特异性： 不与大鼠Adiponectin类似物等反应。
精密度： 板内，板间变异系数均<10%
回收率： 80%-120%
反应时间： 3.5h

Gene ID： 246253
检测原理： Acmec ELISAyabo官网手机版 盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠Adiponectin单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠Adiponectin会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠Adiponectin抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠Adiponectin发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠Adiponectin，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠Adiponectin浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠Adiponectin的浓度。
背景说明： Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin and adiponectin protein of 30 kDa. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, mRNA levels of Adiponectin have been shown to be induced over 100-fold. Studies indicate that Adiponectin enhances the ability of sub-physiological levels of insulin to suppress glucose production, thus linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol-3-kinase (PI3-K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. mRNA levels of Adiponectin have been shown to be significantly reduced in adipose tissue of obese patients with Type 2 diabetes relative to control subjects. Structural similarities to TNFalpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Because Adiponectin is down-regulated in various forms of obesity and its structural similarity to TNFalpha, it is currently being investigated as an important regulator of whole body energy homeostasis.

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